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Expanding the Reach of Recombineering to Environmental Bacteria

GND
117525357X
ORCID
0000-0002-6409-8110
Affiliation/Institute
Institute of Microbiology, Technical University of Braunschweig
Borrero-de Acuña, José Manuel;
ORCID
0000-0001-6649-0389
Affiliation/Institute
Biosystems Engineering Laboratory, Center for Bioinformatics and Integrative Biology, Faculty of Life Sciences, Universidad Andres Bello, Santiago
Poblete-Castro, Ignacio

Broadening the application of recombineering technologies in biotechnologically important bacteria poses significant challenges. Aparicio et al. present a vital breakthrough for efficient single-stranded recombineering by utilizing a thermoinducible system in Pseudomonas putida. This offers a simple genome-editing tool towards creating superior biocatalysts for the synthesis of chemicals and for bioremediation endeavors.

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