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Separation of Dihydro-Isocoumarins and Dihydro-Stilbenoids from Hydrangea macrophylla ssp. serrata by Use of Counter-Current Chromatography

Affiliation/Institute
Institut für Lebensmittelchemie
Wellmann, Johannes; Hartmann, Beate; Schwarze, Esther-Corinna; Hillebrand, Silke; Brueckner, Stephan I; Ley, Jakob;
ORCID
0000-0003-2685-6723
Affiliation/Institute
Institut für Lebensmittelchemie
Jerz, Gerold;
ORCID
0000-0003-3417-6023
Affiliation/Institute
Institut für Lebensmittelchemie
Winterhalter, Peter

Previously, different Hydrangea macrophylla ssp. serrata cultivars were investigated by untargeted LC-MS analysis. From this, a list of tentatively identified and unknown compounds that differ significantly between these cultivars was obtained. Due to the lack of reference compounds, especially for dihydro-isocoumarins, we aimed to isolate and structurally characterise these compounds from the cultivar 'Yae-no-amacha' using NMR and LC-MS methods. For purification and isolation, counter-current chromatography was used in combination with reversed-phase preparative HPLC as an orthogonal and enhanced purification workflow. Thirteen dihydro-isocoumarins in combination with other metabolites could be isolated and structurally identified. Particularly interesting was the clarification of dihydrostilbenoid glycosides, which were described for the first time in H. macrophylla ssp. serrata. These results will help us in further studies on the biological interpretation of our data.

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