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An Immersible Microgripper for Pancreatic Islet and Organoid Research

Affiliation/Institute
Institut für Pharmakologie, Toxikologie und Klinische Pharmazie
Früh, Eike;
Affiliation/Institute
Physikalisch-Technische Bundesanstalt
Bütefisch, Sebastian;
ORCID
0000-0002-7164-5899
Affiliation/Institute
Institut für Mikrotechnik
Gursky, Benjamin;
GND
1218569239
Affiliation/Institute
Institut für Pharmakologie, Toxikologie und Klinische Pharmazie
Brüning, Dennis;
GND
1198905735
Affiliation/Institute
Institut für Mikrotechnik
Leester-Schädel, Monika;
ORCID
0000-0003-2090-6259
Affiliation/Institute
Institut für Mikrotechnik
Dietzel, Andreas;
ORCID
0000-0002-5218-3326
Affiliation/Institute
Institut für Pharmakologie, Toxikologie und Klinische Pharmazie
Rustenbeck, Ingo

To improve the predictive value of in vitro experimentation, the use of 3D cell culture models, or organoids, is becoming increasingly popular. However, the current equipment of life science laboratories has been developed to deal with cell monolayers or cell suspensions. To handle 3D cell aggregates and organoids in a well-controlled manner, without causing structural damage or disturbing the function of interest, new instrumentation is needed. In particular, the precise and stable positioning in a cell bath with flow rates sufficient to characterize the kinetic responses to physiological or pharmacological stimuli can be a demanding task. Here, we present data that demonstrate that microgrippers are well suited to this task. The current version is able to work in aqueous solutions and was shown to position isolated pancreatic islets and 3D aggregates of insulin-secreting MIN6-cells. A stable hold required a gripping force of less than 30 μN and did not affect the cellular integrity. It was maintained even with high flow rates of the bath perfusion, and it was precise enough to permit the simultaneous microfluorimetric measurements and membrane potential measurements of the single cells within the islet through the use of patch-clamp electrodes.

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