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The importance of biofilm formation for cultivation of a Micrarchaeon and its interactions with its Thermoplasmatales host (metabolome data)

Affiliation/Institute
Department of Applied Biology, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany
Krause, Susanne;
Affiliation/Institute
Department of Applied Biology, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany, Institute for Biological Interfaces, Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany
Gfrerer, Sabrina;
Affiliation/Institute
Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
von Kügelgen, Andriko;
Affiliation/Institute
Bioinformatics & Biochemistry, Technische Universität Braunschweig, Braunschweig, Germany, Braunschweig Integrated Centre for Systems Biology (BRICS), Technische Universität Braunschweig, Braunschweig, Germany
Reuse, Carsten;
Affiliation/Institute
Department of Marine Microbiology and Biogeochemistry, NIOZ, Royal Netherlands Institute for Sea Research, Den Burg, The Netherlands
Dombrowski, Nina;
Affiliation/Institute
Department of Marine Microbiology and Biogeochemistry, NIOZ, Royal Netherlands Institute for Sea Research, Den Burg, The Netherlands, Department of Earth Sciences, Faculty of Geosciences, Utrecht University, Utrecht, The Netherlands
Villanueva, Laura;
Affiliation/Institute
Leibniz Institute DSMZ, Braunschweig, Germany
Bunk, Boyke;
Affiliation/Institute
Leibniz Institute DSMZ, Braunschweig, Germany
Spröer, Cathrin;
Affiliation/Institute
Helmholtz-Centre for Environmental Research UFZ, Magdeburg, Germany
Neu, Thomas R.;
Affiliation/Institute
Helmholtz-Centre for Environmental Research UFZ, Magdeburg, Germany
Kuhlicke, Ute;
Affiliation/Institute
Bioinformatics & Biochemistry, Technische Universität Braunschweig, Braunschweig, Germany, Braunschweig Integrated Centre for Systems Biology (BRICS), Technische Universität Braunschweig, Braunschweig, Germany
Schmidt-Hohagen, Kerstin;
Affiliation/Institute
Bioinformatics & Biochemistry, Technische Universität Braunschweig, Braunschweig, Germany, Braunschweig Integrated Centre for Systems Biology (BRICS), Technische Universität Braunschweig, Braunschweig, Germany
Hiller, Karsten;
Affiliation/Institute
Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom, Structural Studies Division, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, United Kingdom
Bharat, Tanmay A. M.;
Affiliation/Institute
Center for Electron Microscopy, University of Regensburg, Regensburg, Germany
Rachel, Reinhard;
Affiliation/Institute
Department of Marine Microbiology and Biogeochemistry, NIOZ, Royal Netherlands Institute for Sea Research, Den Burg, The Netherlands, Department of Cell- and Molecular Biology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden
Spang, Anja;
Affiliation/Institute
Department of Applied Biology, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany, Institute for Biological Interfaces, Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany, Institute of Technical Microbiology, Technical University of Hamburg, Hamburg, Germany
Gescher, Johannes

Micrarchaeota is a distinctive lineage assigned to the DPANN archaea, which includes poorly characterized microorganisms with reduced genomes that likely depend on interactions with hosts for growth and survival. Here, we report the enrichment of a stable co-culture of a member of the Micrarchaeota (Ca. Micrarchaeum harzensis) together with its Thermoplasmatales host (Ca. Scheffleriplasma hospitalis), as well as the isolation of the latter. We show that symbiont-host interactions depend on biofilm formation as evidenced by growth experiments, comparative transcriptomic analyses and electron microscopy. In addition, genomic, metabolomic, extracellular polymeric substances and lipid content analyses indicate that the Micrarchaeon symbiont relies on the acquisition of metabolites from its host. Our study of the cell biology and physiology of a Micrarchaeon and its host adds to our limited knowledge of archaeal symbioses.

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